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B) Empty Chamber Heat Distribution Studies. 4.0 Operate the steam sterilizer and also start the autoclave data logger to record actual temperature within the sterilization chamber with respect to time. 5.0 When the sterilization cycle completes, 1) Collect printout of the sterilizer and preserve as Annexure.
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The short answer is calibration ensures consistent results from a process. Steam sterilization efficacy is highly dependent upon actual temperature. For example, if a steam autoclave is running at 120°C for 15 minutes, the theoretical lethality of that cycle is only 82% of that of a cycle running at 122°C for the same amount of exposure time.
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